Phenotypic and genotypic profile of hospital bacteria isolated from four Moroccan hospitals

During 2011-2013, 189 bacteria from four Moroccans regional hospitals were referred to The National Institute of Hygiene (NIH) to achieve phenotyping and genotyping. Galleries Api20E, Api20NE and Api20Sthap (bioMérieux), were used for Biochemical identification and disks distribution method was used for antibiotic susceptibility testing. Genotyping was carried out by molecular biology by searching genes of resistance (CTX, SHV and TEM) for enterobacteria, and gene mec A for the staphylocoque. among Enterobacteriaceae Escherichia coli was prevalent, followed by pseudomonas aerugenosa, sthaphylococoque, klebsiella pneumonaie and acenitobacter baumanii. The second common phenotype is expanded spectrum lactamase"ESBL». CTX gene was the most predominant for enterobacteria ESBL with an expression about 43.6%. The gene responsible for resistance to Methicillin in our case was mec A which detected in the six strains tested Methicillinresistant S. aureus (MRSA).